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. 2023 Feb 18;14:924. doi: 10.1038/s41467-023-36542-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The CAST transposon is displayed from its left end (LE) to its right end (RE). The genes are colored according to function (green: cas12k, orange: transposase genes, light blue: CAST regulator gene (cvkR in Anabaena 7120), yellow: regions from which non-coding RNA is transcribed, dark gray: cargo genes). On top, the region from the tRNA gene to the tracrRNA is enlarged. The CRISPR array is depicted with its repeats (gray) and spacers (yellow) separated from the 17 nt anchor spacer (blue) downstream of the array next to a truncated repeat sequence (R*; ~12 nt). The scheme is not drawn to scale, but distances of particular interest or mentioned in the text are indicated.