Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 18;14:929. doi: 10.1038/s41467-023-36614-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Electron microscopic appearance of macrophage death in human atherosclerotic plaques. Fragments of a dying macrophage with still recognizable lysosomes (L). Macrophages with normal-looking nucleus (N) and numerous lysosomes (L) containing inclusions of phagocytosed cell remnants. Representative electron microscopic analysis of six different human plaques from one experiment. Scale bar: 5 μm; Scale bar: 2 μm (magnification). b Transmission electron microscopy (TEM) images of a suspected pyroptotic macrophage in human carotid artery atherosclerotic plaques. The arrows indicate pore formation and discontinuity of the cell plasma membrane. Scale bar: 2 μm (right), Scale bar: 1 μm (middle), Scale bar: 500 nm (right); Asterisks indicate cell membrane thickening. Representative electron microscopic analysis of six different human plaques from one experiment. c Immunohistochemical staining of GSDME, caspase 3, IL-1β in human carotid artery compared with control vessels (human normal abdominal artery derived from autopsy) and the protein integrated optical density (IOD)/area between the two groups are shown (mean ± SEM, n = 6). GSDME (**P = 0.004), caspase 3 (**P = 0.004), IL-1β (**P = 0.004). Scale bar: 200 μm; Scale bar: 100 μm (magnification) . d In situ detection of the interaction of GSDME and caspase 3 in human carotid atherosclerotic plaques and control vessels (mean ± SEM, n = 5) using a generalized proximity ligation assay compared with immunohistochemical staining of IL-1β. **P = 0.009. For all panels, P value was determined by two-tailed Mann–Whitney U test. Scale bar: 100 μm; Scale bar: 50 μm (magnification) . Source data are provided as a Source Data file.