Fig. 5. IL-12Rβ2 promotes IFNγ, IL-17A and GM-CSF production by donor T cells in the GI tract.

Lethally irradiated WT and IL-12Rβ2KO B6 mice underwent BMT with 5×10⁶ TCD-BM from FVB mice (Thy1.1⁺) alone (BMA) or plus splenocytes (equal to 0.5×10⁶ T cells) from FVB mice (Thy1.2⁺). Recipient intestines were harvested on day 14 after BMT and analyzed with flow cytometry. Representative dot plots, the average frequencies and absolute counts of IFN-γ⁺, IL17A⁺, and GM-CSF⁺ among gated donor CD4⁺ or CD8⁺ T cells are shown (A and B). The experiments were repeated 3 independent times. 4–5 mice per group were used for each experiment. Statistical data are presented as mean ± 1SD; significance was determined by Student’s t test. * p < 0.05, **p < 0.01 and ***p < 0.001.