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. Author manuscript; available in PMC: 2023 Sep 27.
Published in final edited form as: ACS Nano. 2022 Aug 29;16(9):14792–14806. doi: 10.1021/acsnano.2c05647

Figure 4.

Figure 4.

LNP inhalation produces selective lung transfection. (a) A representative bioluminescent image of isolated organs showing a localized luciferase expression in the lungs. (b) A diagram of mouse lung anatomy showing five lung lobes (left), and ex vivo bioluminescent images of mouse lung lobes 24 h after LNP inhalation (right, n=4). (c) Quantification of total flux in individual lung lobes harvested from BALB/c mouse at 24 h after LNP-Sito/1.5 inhalation at a dose of 100 μg mRNA (n=4). (d) Normalized Nluc expression in mouse lung homogenates harvested from BALB/c mice at 24 h after inhalation of LNP-Chol and LNP-Sito containing Nluc mRNA and various amount of PEG lipid. Data were presented in mean ± standard deviation. ****p<0.001, **p<0.01; significant analysis by two-way ANOVA with Sidak’s multiple comparison test (n=8).