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. Author manuscript; available in PMC: 2023 Sep 27.
Published in final edited form as: ACS Nano. 2022 Aug 29;16(9):14792–14806. doi: 10.1021/acsnano.2c05647

Figure 6.

Figure 6.

Inhaled nLNP transfects airway epithelium without causing acute toxicity. (a) A diagram of Cre-lox recombination in Ai9 transgenic Cre reporter mouse that expresses tdTomato when loxP recombination has occurred. (b-c) Representative immunohistochemistry of Ai9 mouse lung sections displaying tdTomato expression after inhalation of nLNP encapsulating Cre mRNA. tdTomato (red) and nuclei (white) were detected in (b) alveolar spaces and (e) airway bronchioles. 20x magnification. Scale bars refer to 50 μm. (d,e) Clinical chemistry test of mouse sera harvested at 24 h after PBS or nLNP inhalation. Data were presented in mean ± standard deviation. **p<0.01, significant analysis by multiple unpaired t-test (n=3). (f,g) Histopathological analysis of (f) alveolar spaces and (g) bronchial epithelium of murine lungs harvested at 24 h after PBS (left) or nLNP (right) inhalation. Hematoxylin and eosin; 20x magnification. Arrows indicate the bronchial epithelium.