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. 2023 Feb 16;11:e14824. doi: 10.7717/peerj.14824

Figure 3. m6A methylation of exon-intron boundaries prevents intron splicing of KRT4 pre-mRNA in OSCC.

Figure 3

(A) Quantification of KRT4 pre-mRNA containing exon-intron structures by qRT-PCR following RIP performed by METTl3 or METTL14 antibody in HN6 cells. (B) The m6A level of m6A sites in exon 3 (KRT4-E3(4)) and exon 5 (KRT4-E5(6)) of KRT4 pre-mRNA detected by MeRIP in HN6 cells. (C) Levels of junctions of intron 2-exon 3 (KRT4-E(2)3), exon 3-intron 3 (KRT4-E(3)4) and exon 5-intron 5 (KRT4-E(5)6) in KRT4 pre-mRNA detected by qRT-PCR in HN6 cells treated with or without METTL3 and METTL14 siRNA. (D) Levels of KRT4 mRNA in HN6 cells treated with or without METTL3 and METTL14 siRNA. NC, negative control; siMETTL3, METTL3 siRNA; siMETTL14, METTL14 siRNA; E, exon. ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.