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(A) Quantification of KRT4 pre-mRNA containing junctions of intron 2-exon 3 (KRT4-E(2)3), exon 3-intron 3 (KRT4-E(3)4) and exon 5-intron 5 (KRT4-E(5)6) in KRT4 pre-mRNA by qRT-PCR in HN6 cells treated with or without DGCR8 siRNA. (B) Levels of KRT4 mRNA in HN6 cells treated with or without DGCR8 siRNA. NC, negative control; siDGCR8, DGCR8 siRNA; E, exon. ∗∗∗P < 0.001.
