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. Author manuscript; available in PMC: 2023 Feb 20.
Published in final edited form as: Cell Rep. 2023 Jan 4;42(1):111946. doi: 10.1016/j.celrep.2022.111946

Figure 3. Reduced activity in low-responding neurons compensates for hyperactivity.

Figure 3.

(A) Representative traces of calcium transients and corresponding deconvolved spike trace of spontaneous activity from WT and hAPP mice.

(B) Average AUC and the number of deconvolved spikes (spike events) in every 100 s of spontaneous activity from all identified neurons for each animal. n = 10 (WT, 860 neurons) and 9 (hAPP, 780 neurons) mice.

(C) Representative traces of calcium transients and corresponding deconvolved spike trace across the entire imaging session of evoked activity (multiple stimuli) from WT and hAPP mice.

(D and E) Average AUC (top) and the number of deconvolved spikes (spike events, bottom) in every 100 s of evoked activity across multiple stimuli (D). Shown are (left) all identified (740 WT and 434 hAPP neurons), (center) active for any stimulus (high-threshold criteria; 353 WT and 168 hAPP neurons), and (right) weakly responsive for any stimulus (active only when the activity criteria threshold is lowered; 349 WT and 171 hAPP neurons) from n = 8 WT and 6 hAPP mice. p < 0.01 (unpaired Student’s t tests, left and right bottom). Also shown (E) are the average AUC of trial-averaged dF/F0 (3 s) elicited by NI stimuli of all identified neurons (WT = 740, hAPP = 434 neurons; left) and neurons classified as active only when the threshold is lowered (238 WT and 84 hAPP neurons; right) from 8 WT and 6 hAPP mice. Data are mean ± SEM. Circles in the histogram represent individual mouse values.

(F) Proportion of neurons that are not (351 WT and 279 hAPP), weakly (238 WT and 84 hAPP), or highly (151 WT and 71 hAPP) responsive to NI stimuli from all mice. p < 0.001 (chi-square test).