Skip to main content
. 2022 Jun 23;13(1):410–424. doi: 10.1016/j.apsb.2022.06.012

Figure 1.

Figure 1

Chlorella axenic culture and characterization of Chlorella hydrogels. (A) Axenic validation of Chlorella with BG-11 agar plate detected by an inverted fluorescence microscope. Scale bars: 500 μm. (B) Axenic validation of Chlorella with beef extract peptone agar plates detected by colony count. (C) Re-suspending stability of Chlorella in BG-11 medium for 12 h standing, 37 °C, 800 lx. (D) Cytotoxicity of Chlorella (1 × 109 cell/mL) on L-929, HaCaT and HUVEC cells for 24 h (n = 6). (E) SEM photographs of Chlorella hydrogels. Scale bars: 100 μm in 200 ×, 10 μm in 2000 ×. (F) Photographs of Chlorella hydrogels after preparation and Day 30 after storage. (G) Proliferation of axenic Chlorella in BG-11 medium and the hydrogels with BG-11 for 24 h, 37 °C, 800 lx, respectively (n = 6). The concentrations of axenic Chlorella: 1 × 106, 1 × 107, 1 × 108 and 1 × 109 cell/mL. (H) Proliferation of axenic Chlorella in the hydrogels after storage for 30 days, 4 °C, dark (n = 6). (I) Residual NO3– in axenic Chlorella hydrogels after storage for 30 days, 4 °C, dark (n = 6). Data are presented as mean ± SD.