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. 2022 Jun 23;13(1):410–424. doi: 10.1016/j.apsb.2022.06.012

Figure 3.

Figure 3

Glucose and ROS depletion by Chlorella. (A) Glucose remaining in the BG-11 medium (with 33 mmol/L glucose) after 12 h incubation with Chlorella. The concentrations of Chlorella in the medium were selected as 1 × 106, 1 × 107, 1 × 108 and 1 × 109 cell/mL (n = 6). (B) Glucose inside the Chlorella after 12 h incubation (n = 6). (C) Glucose remaining in the lower chamber of the transwell after 12 h incubation with Chlorella hydrogels (n = 6). A–C: 37 °C, dark/10,000 lx (1 h:11 h light/dark)/continuous 800 lx. Dotted lines marked the glucose concentration remaining in the control group. (D–F) The depletion of H2O2 and the inhibition of ·OH–/·O2– generation by Chlorella in BG-11 medium, 37 °C, dark/10,000 lx/800 lx, respectively. (n = 6). (G–I) Effect of Chlorella hydrogels on L-929 cell proliferation, HaCaT migration and transwell migration of HUVEC, 37 °C, dark, respectively. The concentration of H2O2 in the cell culture medium was 1 mmol/L. Scale bar: 200 μm. Data are presented as mean ± SD; ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, marked necessarily.