ROS depletion and anti-inflammation by inactivated-Chlorella. (A–C) The comparison of hydrogels with Chlorella and inactivated-Chlorella on the depletion of H2O2 and the inhibition of ·OH–/·O2– generation, respectively. The concentration of Chlorella was 1 × 106, 1 × 107, 1 × 108 and 1 × 109 cell/mL (n = 6). (D–F) The anti-inflammation function of hydrogels containing inactivated-Chlorella, reflected by the secretion of IL-6 and TNF-α and the production of NO in LPS-induced RAW 264.7 cells (n = 6). (G) Effect of inactivated-Chlorella hydrogels and Chlorella hydrogels on the transwell migration of RAW 264.7 cells. Scale bar: 200 μm. Data are presented as mean ± SD; ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, marked necessarily.