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. 2022 May 25;13(1):157–173. doi: 10.1016/j.apsb.2022.05.019

Figure 2.

Figure 2

PRMT6 promotes lung cancer cell growth. (A) The cell proliferation was determined by cell number counting in H1299 and H460 cells with stable knockdown of PRMT6 (Data represent mean values ± SD from three independent experiments). (B) The colony formation was determined in H1299 and H460 cells with stable knockdown of PRMT6. (C) Tumor growth was compared between xenograft nude mice with H460 cells injected with PRMT6 shRNA virus and control shRNA virus (n = 4). (D) All tumors from nude mouse are shown. (E) Tumor mass in xenograft nude mice with H460 cells injected with PRMT6 shRNA virus and control shRNA virus (n = 4). (F) The arginine methylation levels of 6PGD/ENO1 and 6PGD/ENO1 were analyzed by immunoprecipitation (IP) assay in a representative H460 tumor cells injected with PRMT6 shRNA virus and control shRNA virus. (G) Tumor growth was compared between xenograft nude mice bearing with lung cancer PDX injected with PRMT6 shRNA virus and control shRNA virus (n = 7). (H) All tumors in xenograft nude mouse are shown. (I) Tumor mass in xenograft nude mice bearing with lung PDX tumor injected with PRMT6 shRNA virus and control shRNA virus (n = 7). (J) The arginine methylation levels of 6PGD/ENO1 and 6PGD/ENO1 were analyzed by immunoprecipitation (IP) assay in presentative PRMT6 knockdown lung PDX tumor. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.