Figure 3.

PRMT6 promotes the oxidative PPP flux and glycolysis pathway. (A–D) PRMT6 knockdown and control vector cells were tested for lactate production (A), DNA biosynthesis (B), and NADPH/NADP⁺ ratio (C), as well as ROS level (D). (E) Schematic of oxidative PPP flux and glycolysis pathway flux, illustrating labeling from d-[1,2]-¹³C-glucose. (F) Fractional labeling of metabolites of glycolysis pathway and oxidative PPP flux in PRMT6 knockdown and control vector cells cultured in medium containing d-[1,2]-¹³C-glucose for 15 min. Data represent mean values ± SD from three independent experiments. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001.