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. 2022 May 25;13(1):157–173. doi: 10.1016/j.apsb.2022.05.019

Figure 4.

Figure 4

PRMT6 methylates 6PGD/ENO1 and enhances their activities. (A) Analysis of glycolysis-, and oxidative PPP-related gene expression in PRMT6 knockdown cells and control vector cells. (B) The expression of glycolysis-, and oxidative PPP-related enzymes were determined by Western blotting in HEK293T cells by Flag-pull down assay. (C) 6PGD or ENO1 associates with PRMT6 in vitro. The rFlag-PRMT6 was incubated with recombination GST-taged 6PGD (rGST-6PGD or rGST-ENO1). After GST pull down assay, the interaction of rFlag-PRMT6 protein with rGST-6PGD (or rGST-ENO1) were analyzed by Western blotting. (D) Purified rGST-6PGD was incubated with purified Flag-tagged PRMT6 from HEK293T cells, followed by 6PGD enzyme activity assay. (E) Purified rGST-6PGD was incubated with purified Flag-tagged PRMT6 from HEK293T cells, followed by Western blotting. (F) Purified rGST-ENO1 was incubated with purified Flag-tagged PRMT6 from HEK293T cells, followed by ENO1 enzyme activity assay. (G) Purified rGST-ENO1 was incubated with purified Flag-tagged PRMT6 from HEK293T cells, followed by Western blotting. Data represent mean values ± SD from three replicates of each sample (∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001).