Figure 4.

Elimination of H₂O₂ by NPs. (A–C) The image and morphology of NPs (RP-PU, PP-PU, RPP-PU) in PBS or H₂O₂ were observed by TEM. (D) In vitro release of RPP-PU was observed in PBS or H₂O₂ solution with a concentration of 10, 100, and 1000 μmol/L in PBS. (E) In vitro release of RPP-PU was observed in FBS or H₂O₂ solution with a concentration of 10, 100, and 1000 μmol/L in FBS. (F–I) In vitro release of RP-PU, PP-PU, and RPP-PU was observed in FBS or H₂O₂ solution with a concentration of 10, 100, and 1000 μmol/L in FBS. (J) Determination of residual H₂O₂ at 2 h after NPs incubation. (K) Removal of H₂O₂ by RPP with different concentrations. (L) Removal of H₂O₂ by different materials. (M–P) The ability of NPs to eliminate H₂O₂ in RAW and EC. 1 μg LPS (about 100 nmol/L) induced ROS production in all groups except the negative control group. Images of NPs in eliminating H₂O₂ in RAW (M) and EC (N) and corresponding quantitative Results (O, P). Scale bars = 10 μm. Data are presented as mean ± SD (n = 5). ∗∗P < 0.01, ∗∗∗P < 0.001. NS, not significant.