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In vitro model protein BSA labeling with desthiobiotin probes. (A) Illustration of “click chemistry” based labeling using desthiobiotin-azido probe 15. (B) Illustration of “FTDR” based labeling using desthiobiotin-SH probe 11. (C) PAGE gel results of the protein labeling. “CBB” is Coomassie brilliant blue staining. The first-step installation of the alkyne (A) or acetyl fluoride (B) tags were based on the random conjugation of lysine-NHS chemistry.
