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. 2022 Sep 13;13(1):256–270. doi: 10.1016/j.apsb.2022.09.008

Figure 5.

Figure 5

In vitro biochemical assay and phylogenetic analyses of hydroxylase OxaK. (A) HPLC traces of the OxaK-catalyzed reactions. (B) Structures of the two new OxaK enzymatic products. (C) UPLC–MS analysis of the products of the OxaK-catalyzed reactions. (D) Phylogenetic analyses of OxaK with Fe(II)/α-KG-dependent dioxygenases. (E) Proposed reaction mechanism and substrate scope of hydroxylase OxaK. The chromatograms were monitored at 310 nm.