Figure 3.

Four CRM subsets existed successively and exhibited different functions during ontogeny. (A) Selected categories from GO enrichment analysis of DEGs are expressed as –log₁₀P value adjusted for multiple comparisons. (B) Immunofluorescence staining of Ki67 (left) and Aurora B (right) in cTNT positive neonatal mouse cardiomyocytes directly co-cultured with CRMs from mouse hearts of different ages (None, E18D, P7D, P4W, P1Y). The representative images are shown above and statistical analysis shown below. X axis presents different CRMs treatment. (C) Immunofluorescence staining of Ki67 and Aurora B in neonatal mouse cardiomyocytes co-cultured with CRMs from embryonic or neonatal mice directly or indirectly. The representative images are shown left and statistical analysis shown right. (D) Neonatal mouse cardiomyocytes were directly co-cultured with CRMs from embryonic and neonatal mouse hearts by cell–cell contact, CRMs were stained by F4/80, and the cardiomyocytes were stained by cTNT. For B and C, the data were repeated 3–5 times, similar data were obtained, the representative images are shown. ∗P < 0.05. E18D, P7D, P4W and P1Y mean embryonic Day 18, post-natal 7 days, 4 and 8 weeks, and 1-year-old, respectively.