Figure 2.

Allosteric binding properties of pyrazol-4-yl-pyridine compounds 8–13 at the hM₄ mAChR. (A) Radioligand binding experiments using CHO cells stably expressing the hM₄ in the presence of a K_D concentration of the radiolabeled antagonist [³H]NMS (∼0.1 nmol/L, increasing concentrations of ACh with or without increasing concentrations of 8–13. (B) Affinity parameters and (C) cooperativity parameters of pyrazol-4-yl-pyridine compounds obtained using the ATCM [Eq. (1)]. Values represent the mean ± SEM obtained from three experiments conducted in duplicate (total n = 6).