Figure 5.

In vitro autoradiography of [¹⁸F]12 on rodent, non-human primate (NHP) and human brain tissue sections. (A) Representative autoradiograms of the rat brain in the presence and absence of orthosteric agonist, carbachol. Blocking was performed using an excess of non-radioactive compound 11 (10 μmol/L). Striatal regions were delineated and highlighted with an arrow. Quantification of the radioactive signal is presented for the striatum and in the presence of carbachol. (B) Representative autoradiograms of the mouse brain in the presence and absence of orthosteric agonist, carbachol. Blocking was performed using an excess of non-radioactive compound 11 (10 μmol/L). Striatal regions were delineated and highlighted with an arrow. Quantification of the radioactive signal is presented for the striatum and in the presence of carbachol. (C) Comparison of specificity values for rodents in the presence and absence of carbachol. (D) Regional binding pattern in the rat brain. (E) Representative autoradiograms of the NHP brain and quantification of the radioactive signal. Blocking was performed using an excess of non-radioactive compound 11 (10 μmol/L). (F) Representative autoradiograms of the human putamen and quantification of the radioactive signal. Blocking was performed using an excess of non-radioactive compound 11 (10 μmol/L). (G) Comparison of specificity values for primates in the presence and absence of carbachol.