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. 2022 Apr 9;13(1):142–156. doi: 10.1016/j.apsb.2022.04.003

Figure 4.

Figure 4

Deletion of 17β-HSD7 in macrophages reduced hepatic lipid accumulation. (A) Liver TG, NEFA and TC levels in WT and cKO mice fed with HFD or NCD (n = 7–8). (B) Serum TG, NEFA, TC levels, and (C) LDL-C and HDL-C levels in WT and cKO mice fed with HFD or NCD (n = 7–8). (D) Representative pictures of Oil Red O staining of liver sections (scale bar: 100 μm). (E) Representative pictures of Oil Red O staining of primary hepatocytes co-cultured with BMDMs from WT and cKO mice treated with LOP (left panel, scale bar: 100 μm), and quantitative analysis at OD 570 nm (right panel, n = 3). (F) Representative pictures of Oil Red O staining of AML-12 co-cultured with shControl and shHsd17b7 treated with LOP (left panel, scale bar: 100 μm), and quantitative analysis at OD 570 nm (right panel, n = 3). Data are expressed as mean ± SD. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. 17β-HSD7, 17β-hydroxysteroid dehydrogenase type 7; NEFa, free fatty acid; HDL-C, high-density lipoprotein cholesterol; HFD, high-fat diet; LDL-C, low-density lipoprotein cholesterol; LOP, 100 ng/mL LPS + 250 μmol/L OA + 125 μmol/L PA; LPS, lipopolysaccharides; NCD, normal chow diet; NEFA, non-esterified fatty acid; OA, oleic acid; PA, palmitic acid; TC, total cholesterol; TG, triglyceride.