FIGURE 4.

Induction of apoptosis and cell cycle arrest in hiPSCs but not in iMSCs by BRQ treatment. hiPSCs were cultured until 70% confluence was reached, and iMSCs were treated directly after plating for 7 days. (A) Apoptosis detection in 24 h and 48 h BRQ- and DMSO-treated hiPSCs and 7 days BRQ and DMSO-treated iMSCs. Dissociated cells were collected in cell suspension, stained with FITC labeled Annexin V and propidium iodide (PI) using an Annexin Apoptosis Detection Kit, and analyzed by flow cytometry. n = 3, representative figures. (B) Cell cycle analysis of 24 h and 48 h BRQ- and DMSO-treated hiPSCs, and 7 days BRQ- and DMSO-treated iMSCs using a Cell Cycle Assay kit and analyzed using flow cytometry. n = 3, biological replicates.