Fig. 1.

Schematic representation of plasmid constructs used for genetic transformation of chickpea: a T-DNA of pBK201 harboring cry2Aa gene driven by AraSSU promoter and TobSSU terminator in a twin T-DNA binary vector, b T-DNA of pBin35S2Aa containing cry2Aa gene driven by CaMV35S promoter and nptII gene driven by NOS promoter, LB, RB left and right borders of T-DNA, CaMV35S cauliflower mosaic virus promoter, NOS nopaline synthase promoter and terminator, OCS octopine synthase terminator, AraSSU Arabidopsis thaliana small subunit promoter, TobSSU tobacco small subunit terminator, nptII neomycin phosphotransferase II gene, SCSV1 subclover stunt virus segment 1 promoter, SCSV3 subclover stunt virus segment 3 terminator