Table 1.
Read counts and assembly statistics for the de novo transcriptome assemblies
| Number of reads | Transcripts in assembly | N50 | |||||
|---|---|---|---|---|---|---|---|
| Organism | Phylum | Raw | Processed | Initial | Final | N50 (initial) | N50 (final) |
| Acartia tonsa | Ar | 46728568 | 30183880 (64.6) | 160483 | 22787 (14.2; 42.7*) | 1452 | 1959 |
| Acartia clausii | Ar | 42990921 | 23138097 (53.8) | 159937 | 23313 (14.6; 38.7*) | 1377 | 1897 |
| Calanus helgolandicus | Ar | 19503965 | 17217713 (88.3) | 164611 | 43040 (26.1; 30.2*) | 1366 | 1570 |
| Centropages hamatus | Ar | 21834660 | 18449434 (84.5) | 161672 | 23278 (14.4; 38.8*) | 1573 | 2042 |
| Temora longicornis | Ar | 38035737 | 32327268 (85) | 204478 | 25724 (12.6; 34.3*) | 1611 | 2021 |
| Crangon crangon | Ar | 19496190 | 16749098 (85.9) | 182017 | 22686 (12.5; 43.1*) | 2340 | 2968 |
| Corystes sp. | Ar | 20596859 | 17303099 (84) | 176664 | 28738 (16.3; 28.6*) | 1912 | 2263 |
| Hyperia sp. | Ar | 14434862 | 12339911 (85.5) | 127319 | 17429 (13.7; 21.3*) | 1021 | 1408 |
| Podon leuckartii | Ar | 20965748 | 18577507 (88.6) | 111883 | 21961 (19.6; 53.5*) | 2535 | 2477 |
| Evadne nordmanni | Ar | 38147607 | 32851241 (86.1) | 125959 | 14391 (11.4; 51.5*) | 2861 | 2935 |
| Poecilochaetus sp. | An | 19082838 | 16260294 (85.2) | 272460 | 35984 (13.2; 21.2*) | 1325 | 1807 |
| Magelona mirabilis | An | 16287463 | 13439540 (82.5) | 246407 | 27690 (11.2; 29.1*) | 1358 | 2029 |
| Phoronis muelleri | Ph | 18102502 | 16002988 (88.4) | 169860 | 24094 (14.2; 39.8*) | 2000 | 2532 |
| Oikopleura dioica | Ch | 34115579 | 29875095 (87.6) | 113214 | 19750 (17.4; 49.5*) | 2275 | 2286 |
| Asterias rubens | Ec | 19841332 | 16895994 (85.2) | 127179 | 17918 (14.1; 33.7*) | 1881 | 2520 |
| Rathkea octopunctata | Cn | 17342954 | 15271220 (88.1) | 77799 | 16332 (21; 61.5*) | 2160 | 2574 |
| Phialella quadrata | Cn | 19314077 | 17345509 (89.8) | 77732 | 16145 (20.8; 61.6*) | 2338 | 2626 |
‘Initial’ and ‘Final’ refer to the status of the transcriptome before and after being subjected to an assembly optimization workflow. Numbers in parentheses indicate percentage value with respect to initial/raw numbers; the numbers in the parentheses marked with an asterisk under the column ‘Final’ in ‘Transcripts in assembly’ indicate the percentage of sequences in the final assembly that were identified as being complete open reading frames. N50: contigs of length greater than or equal to the N50 value contain 50% of all bases in the transcriptome. Phylum abbreviations: Ar - Arthropoda, An - Annelida, Ph - Phoronida, Ch - Chordata, Ec - Echinodermata, Cn - Cnidaria. nt: nucleotides