Fig. 2.

PDGFRα+ OPCs are highly proliferative. A. Brightfield images of OPCs in an ideal culture at 1, 3, 5 and 7 DIV and maintenance protocol (written above the images). Scale bar, 20 µm. B. Representative immunofluorescence images (from three independent experiments) of OPCs in standard proliferation media or media supplemented with PDGF-AA (final concentration: 20 ng/mL) labelled with EdU (magenta). Nuclei stained with DAPI. Images taken on an EVOS light microscope, Cy5 light cube. Scale bar, 20 µm. C. Quantification of EdU+ cells as a percentage of DAPI in standard or supplemented proliferation conditions. Data represents mean $\pm$ SEM from three independent experiments, *p < 0.05. Unpaired, two-sided Student's t-test.