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. 2023 Feb 2;10:102051. doi: 10.1016/j.mex.2023.102051
Dulbecco's Modified Eagle's Medium (DMEM) 49 mL

  • B-27 (-vitamin A)

  • 1 mL

  • Penicillin-Streptomycin

  • 500 µL

  • GlutaMAX

  • 500 µL

  • Sodium Pyruvate

  • 500 µL

  • Insulin (0.5 mg/mL)

  • 500 µL

  • SATO supplement

  • 500 µL

  • Trace Elements B

  • 50 µL

  • N-acetyl-l-cysteine

  • 50 µL

  • Forskolin

  • 50 µL

  • Biotin

  • 10 µL

  • PDGF-AA

  • 50 µL

  • bFGF

  • 50 µL