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. Author manuscript; available in PMC: 2023 Feb 20.
Published in final edited form as: Nat Metab. 2022 Aug 18;4(8):1055–1070. doi: 10.1038/s42255-022-00613-w

Extended Data Figure 7. Transient perinatal Pparg overexpression has no apparent long-term effect on APCs differentiation capacity in vitro.

Extended Data Figure 7.

a) 10x brightfield image of indicated cultured APCs at confluence (Day 0), or 2 days (Day 2), or 4 days (Day 4) following the induction of in vitro adipocyte differentiation. APCs obtained from P28 Control and PpargTG mice treated with doxycycline (Dox) from P0.5-P7.5. Oil Red O staining of lipid accumulation and imaging was performed 4 days following the induction of differentiation.

b) mRNA levels of adipocyte-selective genes within differentiated cultures of APCs (Day 4) from P28 Control and PpargTG mice treated with doxycycline (Dox) from P0.5-P7.5. n=4, bars represent ± SEM. * denotes p<0.05 by student t-test.

Exact p values and numbers of repetitions can be found in Source Data Extended Data Figure 7.