Extended Data Fig. 4. Fasting does not alter basic locomotion behavior after fasting.

a. Experimental open field [OF] design for assessment of locomotion of wild type animals after fasting using pharmacological ATX-inhibition (by PF8380) and for assessment of locomotion of fasted animals with cortex-specific ATX-deletion (ATX^Δcortex), of LPA2^-/- mice and of animals with pre-existing increase of synaptic LPA-mediated cortical excitability (PRG-1^R346T/+).

b. PF8380 i.p. application (30mg/kg body weight) significantly reduced LPA-subtype levels in the CSF when compared to vehicle-treated WT animals. Note that some values in the CSF after ATX-inhibition by PF8380 were below the lower level of quantification and set to 0 (n = 4 WT and 6 WT + PF8380 for LPA 16:0, n = 6 WT and WT + PF8380 for LPA 18:1, n = 5 WT and 6 WT+ PF8380 for LPA 18:2 and LPA 20:4, group differences for LPA 16:0 97.6%, for LPA 18:1 99.2%, for LPA 18:2 84.5%, for LPA 20:4 69.9%,Bayesian analysis. Box plots and whiskers show data from min to max, line shows median; group differences represent * >80% or **>90% differences of the mean.

c. Basic spontaneous motor function was not altered after fasting or after PF8380-treatment (n = 13 non-fasted wild type mice [C], 15 fasted wild type mice [F] and n = 15 wild type fasted +PF8380 mice [F + PF8380], one-way ANOVA).

d. Basic spontaneous motor function was not altered in ATX^Δcortex mice when compared to wild type litters under control conditions or after fasting [F] (n = 13 wild type mice [WT and F WT] and n = 12 ATX^Δcortex mice [ATX^{Δcortex and} F ATX^Δcortex], two-tailed t-test).

e. Basic spontaneous motor function was not altered in LPA2^-/- mice when compared to wild type litters under control conditions or after fasting (n = 16 WT litter mice [WT and F WT] and n = 8 LPA2^-/- mice [LPA2^-/- and F LPA2^-/-], t-test) under control conditions or after fasting.

f. Basic spontaneous locomotion was not altered in PRG-1^R346T/+ animals when compared to wild type litters under control conditions or after fasting (n = 8 wild type mice [WT and F WT] and n = 11 PRG-1^R346T/+ mice [PRG-1^R346T/+ and F PRG-1^R346T/+], two-tailed t-test).

Experimental design figures were created with BioRender.com. Dashes show single values, bars show mean and s.e.m.