Figure 7.
Luciferase activity in Sf9_LUC stable cell line without (control) and after exposure to 600 ng of naked dsGFP and dsLUC then conjugated to poly-l-arginine 10 kDa (PLR10) functionalized Au NPs at a NP:dsRNA mass ratio of 5:1 in the case of (a) e-PLR10/Au NPs (e: electrostatic binding) and (b) c-PLR10/Au NPs (c: covalent binding) quenched with BSA (c-PLR10/Au/BSA) or hydroxylamine (c-PLR10/Au/Hyd). Cells were exposed to the pellets redispersed in SF900 II SFM media prior to exposure. Data analyzed using one-way ANOVA with Tukey’s posthoc multiple comparisons. (*) Significantly different at α = 0.05. Error bars were determined from the standard error of the mean of six biological replicates.