Table 1. Experimental Absorption and Fluorescence Properties of iLOV Proteins^a.

	iLOV	iLOV-Q430E	free FMN
maximal absorbance,b nm	370, 448	366, 450	372, 445
extinction coefficient,b mM–1 cm–1	11.9, 15.6	11.8, 12.8	10.0, 12.5
fluorescence wavelength,c,d nm	500 ± 1, 526 ± 1	508 ± 1, 530 ± 1	530 ± 1
relative fluorescence intensityc,d	113 ± 1, 86 ± 1	78 ± 1, 74 ± 1	61 ± 1

^aUV–visible and fluorescence data were recorded in 20 mM pH 8.0 phosphate buffer solution at 15 °C.

^bMaximal absorbance and extinction coefficients are reported for both electronic transitions at the wavelength of maximum absorbance (λ_max).

^cThe fluorescence emission wavelength and intensity are reported for the two vibronic bands, when observed.

^dStandard errors refer to the average of three independent measurements.