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. 2023 Feb 6;14:1114006. doi: 10.3389/fimmu.2023.1114006

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Copyright © 2023 Wang, Zhang, Cheng, Ren, Xu, Li, Wang and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The validation of our bioinformatic predictions by in-vivo and in-vitro experiments. (A) Validation of representative proteins from sequencing. β-Tubulin was used as the control. (B) The quantitative results of Western blotting from (A). (C) Validation of proteins in young (6 weeks) and old (18 months) mice bone. (D) The quantitative results of Western blotting from (C). (E) Representative qRT-PCR quantitation for the marker of inflammatory and SASP. All data were presented as the mean ± SD; *p < 0.05; **p < 0.01, **** p < 0.0001.