Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 20;15:11. doi: 10.1186/s13073-023-01160-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Secretion of TREM2 isoforms. A Western blot analysis of TREM2 isoforms C-terminus (FLAG-tagged) and N-terminus (HA-tagged) in cell extracts (left panel) and media (right panel) of HEK-293T transfected with tagged TREM2 isoforms. The FLAG and HA antibodies were used to detect the C- and N-terminus, respectively. β-Actin and Ponceau staining were used as loading controls for cell extracts and media, respectively. B Immunocytochemistry in permeable and non-permeable conditions (presence and absence of detergents, respectively) was performed in HMC3 cells transfected with TREM2-tagged isoforms. The HA antibody was used to detect all TREM2 isoforms and DAPI to stain cell nuclei. C Scheme of the processing of TREM2 isoforms based on the transfection model