Figure 1.

The ncRNA mutant hid1 exhibits delayed germination under phyB-on conditions. A, Schematic illustration of phyB-on conditions. Seeds were exposed to continuous WL (50 μmol m⁻² s⁻¹) for 1 h before exposure to far-red light (FR) (5 μmol m⁻² s⁻¹) for 5 min (min) to inactivate phyB. The seeds were then irradiated with red light (R) (20 μmol m⁻² s⁻¹) for 5 min to activate phyB before incubation in the dark for the indicated time (5 days). B, Germination rates of WT, hid1, and pHID1:HID1/hid1 (HID1/hid1) seeds under phyB-on conditions. Germination rates were recorded every 24 h after light treatment. Mean ± standard deviation (SD), n = 3. C, Representative images of WT, hid1, and HID1/hid1 seeds at 48 h under phyB-on conditions. Scale bar, 1 mm. D, Germination rates of WT, hid1, mut1/hid1, and mut2/hid1 seeds under phyB-on conditions. Mean ± SD, n = 3. E, Representative images of WT, hid1, mut1/hid1, and mut2/hid1 seeds at 48 h under phyB-on conditions. Scale bar, 1 mm. F, Germination rates of WT, hid1, dSL2/hid1, and dSL4/hid1 seeds under phyB-on conditions. Mean ± SD, n = 3. G, Germination rates of WT, hid1, and OsHID1/hid1 seeds under phyB-on conditions. Mean ± SD, n = 3.