| Strain, strain background (Mus musculus) |
C57BL/6J |
The Jackson Laboratory |
RRID:IMSR_JAX:000664
|
|
| Strain, strain background (M. musculus) |
B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J |
The Jackson Laboratory |
RRID:IMSR_JAX:007909
|
|
| Strain, strain background (M. musculus) |
B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J |
The Jackson Laboratory |
RRID:IMSR_JAX:029684
|
|
| Strain, strain background (M. musculus) |
C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J |
The Jackson Laboratory |
RRID:IMSR_JAX:007900
|
|
| Strain, strain background (M. musculus) |
Tg(Cspg4-DsRed.T1)1Akik/J |
The Jackson Laboratory |
RRID:IMSR_JAX:008241
|
|
| Antibody |
anti-Desmin [Y66] (Rabbit monoclonal) |
Abcam |
Cat#: ab32362 |
1:50 |
| Antibody |
anti-β-III Tubulin [EP1569Y] (Rabbit monoclonal) |
Abcam |
Cat#: ab52623 |
1:200 |
| Antibody |
anti-CD31 [MEC 7.46] (Rat monoclonal) |
Abcam |
Cat#: ab7388 |
1:100 |
| Antibody |
anti-VEGFR2 [EPR21884-236] (Rabbit monoclonal) |
Abcam |
Cat#: ab233693 |
1:500 |
| Antibody |
anti-VEGFA (Rabbit polyclonal) |
Abcam |
Cat#: ab51745 |
1:400 |
| Antibody |
anti-PDGFRβ [Y92] (Rabbit monoclonal) |
Abcam |
Cat#: ab32570 |
1:1000-1:5000 |
| Sequence-based reagent |
B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J F |
The Jackson Laboratory |
PCR primers |
GAA CTG TCA CCG GGA GGA |
| Sequence-based reagent |
B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J R |
The Jackson Laboratory |
PCR primers |
AGG CAA ATT TTG GTG TAC GG |
| Sequence-based reagent |
B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J internal positive control F |
The Jackson Laboratory |
PCR primers |
CAA ATG TTG CTT GTC TGG TG |
| Sequence-based reagent |
B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J internal positive control R |
The Jackson Laboratory |
PCR primers |
GTC AGT CGA GTG CAC AGT TT |
| Sequence-based reagent |
Mouse VEGFA F |
IDT |
PCR primers |
GCAGCGACAAGGCAGACTA |
| Sequence-based reagent |
Mouse VEGFA R |
IDT |
PCR primers |
GGTCCGATGCAAGATCCCAA |
| Peptide, recombinant protein |
Diphtheria toxin |
Sigma |
Cat#: D0564 |
10 ng/g body weight |
| Peptide, recombinant protein |
Dispase II |
Sigma |
Cat#: D4693 |
|
| Peptide, recombinant protein |
Collagenase I |
ThermoFisher |
Cat#: 17018029 |
|
| Peptide, recombinant protein |
DNase I |
Sigma |
Cat#: 10104159001 |
|
| Peptide, recombinant protein |
100× Penicillin-Streptomycin Solution |
Invitrogen/Gibco |
Cat#: 15140–122 |
|
| Commercial assay or kit |
Invitrogen Total Exosome Isolation Reagent (from cell culture media) |
ThermoFisher |
Cat#: 4478359 |
|
| Commercial assay or kit |
SuperSignal West Femto Duration Substrate |
Thermo Fisher Scientific |
Cat#: A38554 |
|
| Commercial assay or kit |
Clontech SMARTer cDNA kit |
Clontech Laboratories |
Cat#: 634925 |
|
| Commercial assay or kit |
NEBNext reagents |
New England Biolabs |
Cat#: E6040 |
|
| Commercial assay or kit |
RNeasy micro kit |
Qiagen |
Cat#: 74004 |
|
| Commercial assay or kit |
SuperScript IV First-Strand Synthesis kit |
ThermoFisher |
Cat#: 18091050 |
|
| Commercial assay or kit |
VEGFA ELISA Kit |
Abcam |
Cat#: ab119565 |
|
| Commercial assay or kit |
BCA protein assay kit |
Abcam |
Cat#: ab102536 |
|
| Commercial assay or kit |
ExoGlow-Protein EV Labeling Kit (Green) |
SBI |
Cat#: EXOGP300A-1 |
|
| Chemical compound and drug |
Tamoxifen |
Sigma |
Cat#: T5648 |
75 mg/kg body weight |
| Chemical compound and drug |
SU5408 |
Abcam |
Cat#: ab145888 |
|
| Software and algorithm |
Sample Size Calculator |
N/A |
https://clincalc.com/stats/samplesize.aspx
|
|
| Software and algorithm |
ImageJ |
NIH |
https://imagej.nih.gov/ij/
|
|
| Software and algorithm |
PANTHER classification system |
N/A |
http://www.pantherdb.org/
|
|
| Software and algorithm |
REVIGO |
Ruđer Bošković Institute |
http://revigo.irb.hr/
|
|
| Other |
Lectin-DyLight 488 |
Vector Laboratories |
Cat#: DL-1174 |
20 μg/ml |
| Other |
Lectin-DyLight 649 |
Vector Laboratories |
Cat#: DL-1178 |
20 μg/ml |
| Other |
Decal Stat Decalcifier |
StatLab |
Cat#: 1212–32 |
Use directly (contains Hydrogen Chloride, Acid mists, strong inorganic) |
| Other |
Antifade Mounting Medium with DAPI |
Vector Laboratories |
Cat#: H-1200 |
Use directly (contains 1 μg/ml of DAPI) |
