Figure 1.

IL-6 promotes the EMT phenotype in EOC cell lines. A2780 cells were treated with exogenous IL-6 (50 ng/ml) for 24 hours, and SKOV3 cells were treated with exogenous IL-6 (10 ng/ml) for 48 hours under normoxic conditions. The expression and localization of E-cadherin and vimentin were then detected by immunofluorescence. The nuclei were stained with DAPI (blue) as an internal reference. E-cadherin and vimentin proteins were visualized by Alexa 488 fluorescent labeling (green) and 549 fluorescent labeling (red), respectively. Scale bars: 100 μm.