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. 2023 Feb 13;2023:8334881. doi: 10.1155/2023/8334881

Figure 4.

Figure 4

HIF-1α inhibits the induction effect of IL-6 on EMT and invasion. (a) Protein levels of EMT markers in transfected SKOV3 cells with repression of HIF-1α after treatment with exogenous IL-6 (10 ng/ml) for 48 hours under normoxic conditions. (b) The invasion ability of transfected SKOV3 cells with repression of HIF-1α was detected by Matrigel invasion assay after treatment as described for (a). Scale bars: 100 μm. In each experiment, the numbers of cells that penetrated the membrane were counted in five microscopic fields per filter. The invasion cell counts are presented as the mean ± SD of three independent experiments. ∗P < 0.05 compared with the SKOV3/scramble shRNA group and #P < 0.05 compared with the IL-6-treated SKOV3/scramble shRNA group by one-way ANOVA with LSD multiple comparison test.