Extended Data Fig. 10. Assessment of Indels from extracardiac tissues derived from mice treated with AAV9-Cas9 nuclease.
Indels were measured by high-throughput sequencing of PCR products amplified from the targeted Myh6 region in gDNA extracted from the liver, lung, gonad and skeletal muscle (quadricep) of mice treated with AAV9-Cas9 nuclease (n = 2-3 per dose) and from untreated mice (n = 3). Reads were aligned to the target site by CRISPResso2. CRISPResso2 quantification of indels is plotted for each sample.