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. 2022 Dec 20;25(3):706–720. doi: 10.1007/s12094-022-02976-8

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — ZBTB4 inhibited proliferation and metastasis in Ishikawa cells in vitro. A The protein expression of ZBTB4 after overexpression and knockdown of ZBTB4. B the effect of ZBTB4 on the proliferation of Ishikawa cells. (i) Overexpression of ZBTB4 inhibited the proliferation of the Ishikawa cell line. (ii) Downregulation of ZBTB4 promoted Ishikawa proliferation. C The effect of ZBTB4 on colony formation of Ishikawa. (i) Knockdown of ZBTB4 facilitated the colony formation of Ishikawa cells. (ii) Overexpression of ZBTB4 did the opposite effect. D Effect of ZBTB4 on migration and invasion ability of Ishikawa cell line. *p < 0.05, **p < 0.01, ***p < 0.001