Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 7;14:920548. doi: 10.3389/fendo.2023.920548

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Yan, Wu, Sun, Fang, Zhang, Zhang, Zhang, Wang, Yang, Yang, Dong, Zhang, Ye, Song and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Isl2a mutants show reduced thyroid function. (A) The assessment of tg expression in isl2a mutants at 3 dpf and 5 dpf via whole-mount in situ hybridization. At 5 dpf, isl2a^-/- zebrafish had lower expression of tg and tshba as denoted by black arrows and arrowhead, respectively. Scale bar = 50 µm. (B) qRT-PCR analysis of the total expression of tg, slc5a5, tpo, tshba, and cga in isl2a^+/+ and isl2a^-/- larvae at 5 dpf. Error bars represent ± SD (n = 5, 15 fish per tube). (C) Both EGFP (green) and EdU immunoreactivity (red) were present in the thyroid of 120 hpf Tg (tg:egfp) wild-type (n = 7) larvae, whereas nearly no EdU-positive cells were detected within the tg domain in isl2a^-/- larvae (n = 6). There were also no TUNEL-positive cells (red) in the tg domain at 127 hpf. Scale bar = 500 µm. (D) Thyroxine (T4) and 3,5,3′-triiodothyronine (T3) levels in the zebrafish body at 44 dpf. Error bars represent ± SD (n = 4). Asterisks indicate significant differences between isl2a^+/+ and isl2a^-/- groups (*P < 0.05, **P < 0.01).