Figure 5.

Comparison between puff latencies theoretically predicted with the rate of IP₃ release θ = 250 μm²s⁻¹ and observed by Dickinson et al.¹¹. Panels (A and B) show minimal first puff latencies and mean latencies simulated with different values of the effective diffusion coefficient for IP₃, D_I. All points correspond to a 200 ms flash. Plain symbols are theoretical predictions while empty symbols are the experimental observations of Dickinson et al.¹¹. Lines are drawn between simulation results. Simulations are performed in a 2D ellipse-shaped system with the spot of IP₃ release being a 0.25 μm radius disk at one extremity of the simulated cell. For each theoretical point, 50 independent simulations were run. Error bars indicate ± SEM. For minimal first puffs, the 50 simulations were divided in 10 groups of 5 simulations among which the minimal first puff was considered.