Table 5.
Appressorium turgor pressure assays of the wild type, ΔMopdx1 mutant, and the complement strains with or without 10-4 mg/mL of VB6 at 14 or 24 hpi, respectively.
| strains | Appressorium collapse rate (%) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 14 hpi | 24 hpi | |||||||||||||||
| -VB6 | +VB6 | -VB6 | +VB6 | |||||||||||||
| 1 M | 2M | 3M | 4M | 1 M | 2 M | 3 M | 4 M | 1 M | 2 M | 3 M | 4 M | 1 M | 2 M | 3 M | 4 M | |
| Guy11 | 18.7 ± 2.5a | 63.3 ± 5.5b | 78.7 ± 7.6b | 85.3 ± 1.5b | 19.0 ± 3.6b | 62.0 ± 3.6b | 77.0 ± 4.5b | 84.0 ± 1.0b | 19.3 ± 1.5a | 52.3 ± 3.8b | 62.7 ± 1.5b | 80.7 ± 7.5b | 21.7 ± 3.5b | 54.0 ± 5.3b | 62.0 ± 2.6b | 78.0 ± 6.1b |
| ΔMopdx1-31# | 7.0 ± 2.0b | 73.7 ± 5.5a | 88.0 ± 3.0a | 94.0 ± 3.6a | 18.0 ± 2.0b | 61.7 ± 5.5b | 79.7 ± 4.5b | 85.7 ± 0.6b | 7.3± 1.5b | 64.0 ± 2.6a | 76.0 ± 6.0a | 90.0 ± 3.0a | 19.0 ± 1.0b | 54.7 ± 3.1b | 63.0 ± 1.0b | 76.0 ± 3.6b |
| ΔMopdx1-43# | 9.7 ± 3.2b | 72.7 ± 4.1a | 87.3 ± 4.5a | 94.7 ± 1.5a | 21.0 ± 2.0b | 62.3 ± 2.1b | 77.3 ± 2.1b | 83.0 ± 2.0b | 8.0 ± 5.2b | 63.7 ± 3.8a | 79.7 ± 1.5a | 91.7 ± 1.5a | 18.7 ± 3.2b | 43.7 ± 4.0b | 63.7 ± 3.8b | 82.0 ± 2.6b |
| ΔMopdx1/MoPDX1 | 20.0 ± 2.0b | 63.0 ± 7.8b | 75.7 ± 3.5b | 84.3 ± 2.5b | 20.3 ± 2.5b | 63.7 ± 3.2b | 76.0 ± 2.0b | 84.7 ± 0.6b | 20.7 ± 2.5a | 51.0 ± 2.0b | 63.3 ± 1.5b | 81.0 ± 1.0b | 20.3 ± 2.5b | 57.0 ± 3.0b | 62.7 ± 2.1b | 81.7 ± 2.5b |
Concentrations of 1-4 M of glycerol were used to analyze incipient cytorrhysis with or without 10-4 mg/mL of VB6 treatments. At least 100 appressoria were counted for each concentration. ±SD was calculated from three repeated experiments and lowercase indicates statistically significant differences (LSD and Student-Newman-Keuls test, p<0.05).