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. 2023 Feb 20;13:2953. doi: 10.1038/s41598-023-28798-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Study design. (A) Experimental timeline. An intrastriatally implanted cannula connected to a minipump releasing vehicle (PBS) or CDNF was installed subcutaneously at 11 weeks of age of N171-82Q mice. Minipumps were removed at 15 weeks. Behavioral tests were conducted twice per week. Animals were sacrificed for collecting tissues at 15 weeks of age for N171-82Q male mice and 16 weeks for N1721-82Q female mice. (B) Scheme of cannula placement (A/P + 0.86; M/L − 1.8; D/V − 3.0).