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. 2023 Feb 20;13:2953. doi: 10.1038/s41598-023-28798-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EM48-positive staining and intranuclear inclusions in the N171-82Q mouse model after chronic injection of CDNF or PBS. WT animals did not show the presentation of mHtt inclusions. The density of EM-positive nuclear staining and number of inclusions in the striatum (A), neocortex (B) and piriform cortex (C) of N171-82Q mice. Quantification analysis was performed at week 15 of life for males and 16 for females. Values are means ± SEM. N number per group = 5–7 (females) and 10–12 (males).