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. Author manuscript; available in PMC: 2023 Feb 21.
Published in final edited form as: Cell Rep. 2023 Jan 10;42(1):111983. doi: 10.1016/j.celrep.2022.111983

Figure 2. Env recognition at the surface of HIV-1-infected primary CD4+ T cells and autologous macrophages by a panel of bNAbs.

Figure 2.

Staining of CD4+ T cells and macrophages infected with HIV-1AD8 panel of viruses (WT, Nef-defective [N–], Vpu-defective [U–], or both Nef and Vpu [N–U–] and CD4BS mutant [D368R] and N-U-D368R mutant) with (A) 2G12 a conformation independent antibody; (B) the V1V2-apex antibody PG9; V3glycan antibodies (C) PGT121 and (D) 10.1074; CD4BS antibodies (E) 3BNC117 and (F) VRC03; (G) PGT151, which targets the gp120-gp41 interface; and the (H) anti-MPER antibody 10E8. Values represent fold change in MFI relative to mock. Error bars indicate mean ± SEM.Statistical significance was tested using two-way ANOVA with Holm-Šídák’s multiple comparisons test (*p < 0.05; **p < 0.001; ***p < 0.0001; ns, non-significant). Shown are data from 6 donors.