Fig. 2.

Effective neutralization of IFN-γ signaling with anti-IFN-γ autoAbs in vitro. The inhibition of IFN-γ-induced STAT1 phosphorylation in THP-1 cells treated with serum samples from anti-IFN-γ-autoAb-positive, anti-IFN-γ-autoAb-negative patients or HC subjects. a The representative histograms of the inhibition of IFN-γ-induced PE (phycoerythrin)-STAT1 phosphorylation in THP-1 cells treated with serum samples from anti-IFN-γ-autoAb-positive, anti-IFN-γ-autoAb-negative patients, or HC subjects. b Comparison of pSTAT1 proportion after inhibition with serum from three groups of subjects. c Anti-IFN-γ-autoAb levels were negatively associated with STAT-1 phosphorylation (ratio). Spearman’s nonparametric test was used in the correlation analysis. Data are presented as a logarithmic scale and box-plot diagrams, with the box encompassing the 25th percentile (lower bar) to the 75th percentile (upper bar). The horizontal line within the box indicates the median value respectively for each group. *p value < 0.05, ***p value < 0.001, determined by the Kruskal–Wallis test with a post-hoc Dunn’s test