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. 2023 Jan 28;26(2):106080. doi: 10.1016/j.isci.2023.106080

Figure 7.

Figure 7

AMG510 modifies Cys288 of KEAP1 and induces the nuclear accumulation of NRF2 in vivo

(A) MS/MS spectrum of the AMG510-modified EILQSDSR from trypsin digested H358 cells after treatment with AMG510.

(B) Western blotting showed the effect of AMG510 on the nuclear and cytoplasmic fraction of NRF2 protein of H358 cells.

(C) Quantitative comparison of peptide-IP and western blot analysis on the activation of the covalent modification of KEAP1Cys288 and nuclear accumulation of NRF2.

(D) Western blotting showed the effect of AMG510 on the nuclear of NRF2 protein of two NSCLC cell lines, including HCC827 (KRAS WT) and A549 (KRAS G12S).