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. 2023 Feb 10;27(3):72. doi: 10.3892/mmr.2023.12959

Figure 3.

Figure 3.

miR-29a-3p inhibits HIF-1α expression by targeting the PI3K/AKT pathway. (A) A schematic diagram of the binding site of miR-29a-3p to the PI3K gene and the PI3K mutation site was predicted by TargetScan. (B) Dual luciferase gene reporter assay was used to verify the targeting negative regulatory effect of miR-29a-3p on PI3K, ****P<0.0001. The expression level of PI3K was analyzed by (C) quantitative PCR and (D) western blotting after U251 cells were transfected with miR-29a-3p mimics and inhibitor, *P<0.05, ***P<0.001. (E) Effect of miR-29a-3p on the expression levels of the PI3K downstream molecules AKT, p-AKT and HIF-1α, *P<0.05, **P<0.01. All data were expressed as the mean ± standard deviation (n=3). miR, microRNA; HIF-1α, hypoxia-inducible factor 1; NC, negative control; UTR, 3′ untranslated region; wt, wild-type; mu, mutant; p-, phosphorylated.