Table 2.
Statistics summary for the 17 Chloroflexi MAGs recovered in this study
| Reactor | Genome | Genome size (bp | CDSa | Completenessb/ Contamination | Contigs | N50 (bp) | 16S/23S/5Sd | tRNAa | %GC | Coverage | Taxonomic annotationc | Closest placement ANI % |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activated sludge | RH21 | 5,657,355 | 5097 | 85.59/0 | 655 | 12,275 | 1(922nt)/1(492nt)/1 | 4 5 | 56.34 | 8.2 | c_Anaerolineae;o_SBR1031;f_A4b;g_OLB15 | 76.07 |
| RH38 | 5,163,972 | 4600 | 87.29/1.73 | 1167 | 5326 | 1(805nt)/1(342nt)/2 | 32 | 64.57 | 8.6 | c_Anaerolineae;o_Caldilineales;f_Caldilineaceae | N/A | |
| RH43 | 9,980,378 | 8014 | 90.86/4.7 | 129 | 94,438 | 1(807nt)/1/1 | 44 | 55.88 | 3.03 | c_Anaerolineae;o_Caldilineales;f_Caldilineaceae;g_CFX5 | 76.3 | |
| RH52 | 4,922,116 | 3510 | 96.79/1.1 | 201 | 35,320 | 1(62nt)/1/1 | 57 | 73.22 | 20.4 | c_Anaerolineae;o_UCB3;f_UCB3;g_UCB3;s_UCB3 sp003576755 | 99.2 | |
| Methanogenic | MO16 | 3,073,917 | 2871 | 86.36/3.36 | 387 | 11,990 | 1(1118nt)/0/0 | 35 | 58.98 | 10 | c_Anaerolineae;o_Anaerolineales;f_Anaerolineaceae;g_Longilinea | 79.35 |
| MO53 | 4,940,107 | 4005 | 87.27/0.91 | 227 | 44,487 | 1(337nt)/1(370nt)/1 | 45 | 58.31 | 13.5 | c_Anaerolineae;o_B4-G1;f_SLSP01 | N/A | |
| MO66 | 4,384,884 | 3803 | 92.73/3.36 | 478 | 14,973 | 0/0/0 | 44 | 64.91 | 14.7 | c_Anaerolineae;o_UBA3071;f_CG2-30–64-16 | N/A | |
| MO118 | 3,352,740 | 2734 | 90.18/2.73 | 86 | 51,993 | 1(847nt)/1(337nt)/1 | 38 | 41.05 | 13.2 | c_Anaerolineae;o_Anaerolineales;f_Anaerolineaceae;g_Flexilinea | 83.18 | |
| Anammox | AMX9 | 4,128,696 | 3450 | 98.18/0 | 54 | 178,058 | 1(800nt)/1(617nt)/1 | 48 | 62.69 | 32.7 | c_Anaerolineae;o_SBR1031;f_A4b;g_OLB13;s_OLB13 sp001567485 | 99.66 |
| AMX14 | 3,390,571 | 3072 | 92.73/0.91 | 124 | 43,811 | 1(329nt)/0/0 | 41 | 56.87 | 25.1 | c_Anaerolineae;o_Anaerolineales;f_EnvOPS12;g_OLB14;s_OLB14 sp900696595 | 99.72 | |
| AMX15 | 3,646,596 | 3399 | 90.91/0.18 | 38 | 293,047 | 1(534nt)/2/1 | 42 | 54.08 | 160.3 | c_Anaerolineae;o_Anaerolineales;f_EnvOPS12;g_UBA12294;s_UBA12294 sp003577395 | 95.27 | |
| AMX39 | 2,675,910 | 2460 | 90.91/0.91 | 18 | 265,768 | 1(445nt)/0/0 | 48 | 52.31 | 19.8 | c_Anaerolineae;o_Anaerolineales;f_EnvOPS12;g_UBA12294;s_UBA12294 sp002050275 | 99.2 | |
| AMX47 | 2,814,255 | 2926 | 91.21/0 | 319 | 11,859 | 1(1498nt, 1518nt)/1/1 | 42 | 66.7 | 10.4 | c_Dehalococcoidia;o_UBA2991;f_UBA2991;g_UCB2 | 82.41 | |
| AMX55 | 2,990,515 | 2772 | 92.73/1.27 | 61 | 69,781 | 1(1509nt)/1(317nt)/1 | 40 | 60.81 | 15.1 | c_Anaerolineae;o_Anaerolineales;f_EnvOPS12;g_UBA7227;s_UBA7227 sp002473085 | 98.72 | |
| AMX56 | 4,499,265 | 3993 | 92.42/1.09 | 96 | 86,147 | 3(1218–1493)/2/2 | 58 | 63.48 | 54.1 | c_Anaerolineae;o_Caldilineales;f_J102 | 76.67 | |
| AMX57 | 4,700,319 | 4113 | 92.73/0 | 236 | 29,630 | 1(703nt)/1(461nt)/1 | 46 | 60.87 | 13.6 | c_Anaerolineae;o_SBR1031;f_A4b;g_GCA-2702065 | 75.87 | |
| AMX68 | 4,329,913 | 4110 | 92.73/0.91 | 70 | 171,406 | 1(195nt)/1(219nt)/1 | 46 | 53.34 | 15.8 | c_Anaerolineae;o_Anaerolineales;f_EnvOPS12;g_OLB14 | 79.8 |
N/A Not assigned
aAs predicted using Prokka (details in Methods section)
bGenome quality estimates from CheckM (details in Methods section)
cTaxonomic assignments from GTDB-Tk (details in Methods section)
dSequences obtained from Barrnap (details in Methods section)