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. 2023 Feb 7;15:1019173. doi: 10.3389/fnmol.2022.1019173

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Copyright © 2023 Lee, Song, Hwang, Baek, Baek, Kim, Park, Choi, Pyo and Cho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of very early and delayed exposure to EE on protein expression in the extrinsic apoptosis pathway in the cerebral cortex and the hippocampus. (A,C) Representative WB images from cerebral cortex (A) and hippocampus (C) obtained after very early EE exposure. (B,D) Quantification of Fas, FADD, and cleaved Caspase-8 levels in the cerebral cortex (B) and the hippocampus (D) (n = 4 per group). (E,G) Representative WB images from cortical (E) and hippocampus (G) obtained after delayed EE exposure. (F,H) Quantification of Fas, FADD, and cleaved Caspase-8 in the cerebral cortex (F) and hippocampus (H) (n = 4 per group). All statistical comparisons were performed via Student’s t-tests. Data represented are means ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001. EE, environmental enrichment; WB, western blot.