TABLE 1.

Primers and probe sequences used for SARS-CoV-2 RNA quantitation

Target	Primers and probe sequences (5′–3′)a	Cycling temp and time	Reference
SARS-CoV-2	HKU-ORF1-F: TGGGGYTTTACRGGTAACCT	25°C, 2 min	69
ORF1ab	HKU-ORF1-R: AACRCGCTTAACAAAGCACTC	50°C, 15 min
	HKU-ORF1-P: FAM-TAGTTGTGATGCWATCATGACTAG-BHQ1	95°C, 2 minb
		95°C, 3 secb
		60°C, 30 secb

^aF, forward; R, reverse; P, probe.

^b40 cycles of amplification.